Journal: Materials Today Bio

Article Title: Human iPSC-EV-loaded nanofiber stent coatings accelerate vascular repair by enhancing EGFR/HIF-1α signaling and suppressing ROCK1-mediated remodeling

doi: 10.1016/j.mtbio.2026.103564

Figure Lengend Snippet: Characterization of human induced pluripotent stem cells (iPSCs) and iPSC-derived extracellular vesicles (EVs). (A) Phase-contrast microscopy image of the IBMS-iPSC-02-07 line, exhibiting typical stem cell morphology with tightly packed colonies, well-defined borders, and a high nuclear-to-cytoplasmic ratio. Scale bar: 50 μm. (B) Western blot analysis confirming the expression of key pluripotency transcription factors OCT4, SOX2, and NANOG. Tubulin served as the loading control. (C) Immunofluorescence staining demonstrating the nuclear localization of pluripotency markers OCT4 and SOX2 (red). The cytoskeleton was stained with F-actin (green) and nuclei were counterstained with DAPI (blue). Scale bar: 75 μm. (D) Validation of EV isolation purity by Western blot. Isolated EVs were enriched for the exosomal markers CD63 and HSP70 but were negative for the endoplasmic reticulum marker Calnexin compared to whole-cell lysates (Cell lysate), confirming the absence of cellular contamination. (E) Transmission electron microscopy (TEM) image revealing the characteristic cup-shaped morphology and lipid bilayer structure of the EVs. Scale bar: 200 nm. (F) Nanoparticle tracking analysis (NTA) determining the particle size distribution and concentration (2.45 × 10 11 particles/mL), showing a size range consistent with small EVs.

Article Snippet: Primary antibodies used for Western blotting and immunofluorescence were categorized as follows: Pluripotency markers: Nanog (1:1000, A3232, ABclonal), OCT4 (1:1000, ab19857, Abcam), and SOX2 (1:1000, ab97959, Abcam).

Techniques: Derivative Assay, Microscopy, Western Blot, Expressing, Control, Immunofluorescence, Staining, Biomarker Discovery, Isolation, Marker, Transmission Assay, Electron Microscopy, Concentration Assay

Journal: Stem cell research

Article Title: Generation of two induced pluripotent stem cell lines from Marfan syndrome patients carrying FBN1 mutations

doi: 10.1016/j.scr.2026.104087

Figure Lengend Snippet: Characterization and validation.

Article Snippet: Undifferentiated iPSC state marker , Rabbit Anti-Nanog , 1:100 , Proteintech, Cat #142951–1-AP , RRID:AB_1607719.

Techniques: Biomarker Discovery, Morphology, Analysis, Immunofluorescence, Staining, Expressing, Stability, Whole-Genome Sequencing, DNA Sequencing, Sequencing, Software, Mutagenesis, Next-Generation Sequencing, Southern Blot, Reverse Transcription Polymerase Chain Reaction, Screening, Genotyping, Tissue

Journal: Stem cell research

Article Title: Generation of two induced pluripotent stem cell lines from Marfan syndrome patients carrying FBN1 mutations

doi: 10.1016/j.scr.2026.104087

Figure Lengend Snippet: Reagent details.

Article Snippet: Undifferentiated iPSC state marker , Rabbit Anti-Nanog , 1:100 , Proteintech, Cat #142951–1-AP , RRID:AB_1607719.

Techniques: Marker

Journal: Stem cell research

Article Title: Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients with TTN mutations

doi: 10.1016/j.scr.2026.104065

Figure Lengend Snippet: Reagents details.

Article Snippet: Undifferentiated hPSC state marker , Rabbit Anti-Nanog , 1:200 , Proteintech, Cat #14295-1-AP , AB_1607719.

Techniques: Immunocytochemistry, Marker